Arbetet resulterade i prop. 1979/80.'10 om kontroll av hybrid—DNA-teknikens av propositionen hemställde socialut- skottet i betänkandet SoU 1979/80:18 att
Drygt 80 män har undersökts hittills och under 2016 kommer fler att omfattas. Female Sexual Function Index (FSFI)-formulär respektive International Index of och följaktligen Ku70 och fosforylerat DNA-PKcs fortsatt vara höga i kluster av
Indeed, Ku70/80 has been proposed to perform at least two functions at telomeres. First, it protects telomeres ends from degradation, and second it regulates telomere length, possibly through a functional interaction with the telomerase holoenzyme [5, 6]. However, how Ku70/80 operates at telomere seems to differ among species. Ku70/80 associates with telomeric DNA ( 13) and binds to TRF1 and TRF2 ( 14, 15). Moreover, Ku has been reported to associate with hTERT and telomerase activity ( 16). However, the precise biological consequence, the biochemical nature of these interactions and the role of DNA-PK in telomere maintenance remain unknown. In summary, this study highlights an unexpected and important function of Ku70: In addition to repairing DNA damage in the NHEJ pathway, Ku70 also regulates pancreatic β-cell proliferation.
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ENSP00000300145 ENSG00000166896 ensHS ens Ku70-binding protein 3. av P Håkansson · 2006 — regarding the function of an alternative mammalian RNR small subunit, and on the role of is the binding of the end-binding Ku70/Ku80 complex, leading to the. av J Alfredsson Timmins · 2009 — forms of the HP1 proteins that perform slightly different functions (Lomberk et al., 2006 choring of the telomeres to the NM is facilitated by Ku70, Esc1, members of the Sir protein Hum Genet 80, 235-46. Cremer, T. and Protection of Kidney Function with Human Antioxidation Protein Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in By agreement with the company, the product's function will not be exposed in the report. of CUX1 with some of these proteins: REV1, PARP1 and Ku70/Ku80.
Ku70 and Ku80 form a heterodimer called Ku that is well known for its role in repairing DNA double-strand breaks by nonhomologous end joining (NHEJ) (33). The
Pace et al The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. Here, we describe intramolecular interactions of the Ku70 C‐terminal domain, known as the SAP domain. Ku70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Ku70 and 50µl of protein G magnetic beads (+).
Recombinant Human Ku70 & Ku80 Heterodimer Protein (Met1-Ile732 & Met1-Asp609) CT018-H07B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this Human Ku70 & Ku80 Heterodimer protein for relevant bioassay and related research.
3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure. The Ku70/80 heterodimer rapidly responds to DSBs, stimulating recruitment of downstream NHEJ factors to protect, process, and ligate broken DNA ends. Work in our lab has shown that a D192A/D195R mutation in helix five of the Ku70 von Willebrand A (vWA) domain leads to an NHEJ defect. However, little is known about the function of this region in We propose a structural model of the functions of the subunit‐specific C‐terminal domains in the context of the full‐length Ku70–Ku80 protein . The Ku70‐SAP domain changes its position on DNA binding from the base of the α/β domain of Ku80 to a position that might be proximal to the ring in the side of Ku that faces the continuous DNA. The heterodimer Ku70/80 plays a critical role in DNA repair and cell death induction after damage. The aberrant expression and localization of these proteins fail to control DNA repair and apoptosis. sClusterin is the Ku70 partner that sterically inhibits Bax-dependent cell death after damage in some pathologic conditions.
Here we report that KU70 and DNA-PKcs unexpectedly function together during the induction of apoptosis after exposure to high levels of etoposide. In the presence of 100 microM etoposide, apoptosis was induced within 1 h in wild type DT40 cells but not in KU70 (-/-) and DNA-PKcs (-/-/-) cells. Concomitantly, DNA-bound Ku70/80 proteins serve as a docking platform for sequential binding of C-NHEJ components and for an array of proteins mediating the DNA-damage response. Further, Ku70/80 have been reported to coordinate DSB repair with cell cycle arrest, pathway choice, and, if needed, apoptosis.
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It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
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Apoptosis is a key tumor suppression mechanism that can be initiated by activation of the proapoptotic factor Bax. The Ku70 DNA end-joining protein has recently been shown to suppress apoptosis by sequestering Bax from mitochondria. The mechanism by which Bax is regulated remains unknown. Here, we identify eight lysines in Ku70 that are targets for acetylation in vivo. Five of these, K539
Au WW and Henderson BR: BARD1 regulates BRCA1 apoptotic function by a av P Umate · 2011 · Citerat av 90 — The role of these motifs in ATP-binding, ATP-hydrolysis (ATPase), RNA A total of 31 DNA helicases (like RecQ members, KU70, MCM proteins, DNA helicase INO80 complex homolog 1 and 2 RuvB-like helicases, Pif1, SWISSPROT:Q9UK80 ENSP00000289865 ENSG00000143258 ensHS ens Ubiquitin ENSG00000117691 ensHS ens secreted protein of unknown function. ENSP00000300145 ENSG00000166896 ensHS ens Ku70-binding protein 3. av P Håkansson · 2006 — regarding the function of an alternative mammalian RNR small subunit, and on the role of is the binding of the end-binding Ku70/Ku80 complex, leading to the. av J Alfredsson Timmins · 2009 — forms of the HP1 proteins that perform slightly different functions (Lomberk et al., 2006 choring of the telomeres to the NM is facilitated by Ku70, Esc1, members of the Sir protein Hum Genet 80, 235-46. Cremer, T. and Protection of Kidney Function with Human Antioxidation Protein Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in By agreement with the company, the product's function will not be exposed in the report. of CUX1 with some of these proteins: REV1, PARP1 and Ku70/Ku80.